X线不同剂量照射诱发C6细胞凋亡的研究

INDUCTION OF APOPTOSIS OF C6 GLIOMA CELLS BY DIFFERENT DOSES OF X-RAY IRRADIATION

为研究体外X线不同剂量照射C6细胞可否诱发其凋亡 ,应用大鼠C6胶质瘤细胞系 ,以 15、2 0、30及 40GyX线分别照射体外C6细胞 ,提取DNA进行琼脂糖凝胶电泳 ,观察其细胞凋亡发生的情况。结果显示 ,15、2 0及 30Gy组在照射后 2h便出现明显的DNA降解 (“梯形结构”) ,其中 15Gy组持续到 8h ,2 0、30Gy组持续到 2 4h。但对照及 40Gy组无明显“梯形结构”出现 ,后者DNA小片段条带明显 ,表明该组细胞坏死明显。提示X线不同剂量照射体外C6细胞可诱使其早期产生凋亡 ,但若剂量增大 (如 40Gy)则可致其坏死。

To evaluate whether different doses of X ray irradiation can induce apoptosis of rat C6 glioma cells. A rat C6 glioma cell was irradiated with X ray in different doses in vitro. Apoptosis was identified by the classical “ladder” pattern (oligonucleosome sized fragments) in DNA agarose gel electrophoresis. Results: The results showed that significant apoptosis was found in 15, 20 or 30 Gy groups at 4 hours and 1 day after X ray irradiation compared with control, whereas this phenomenon could only be seen vaguely in the 40 Gy group. The latter group showed more necrosis than apoptosis. It suggested that the apoptotic mode of cell death and necrosis may represent the response in this X ray irradiated rat C6 glioma cells in vitro.