腺嘌呤制备大鼠慢性肾功能损伤的模型

Adenine-induced Chronic Renal Function Impairment in Rats

背景与目的:利用腺嘌呤制备大鼠慢性肾功能损伤的模型。材料与方法:雄性SD大鼠16只,随机分为正常对照组和慢性肾功能损伤模型组,每组8只。对照组动物灌胃蒸馏水,模型组动物灌胃腺嘌呤悬液100 mg/(kg·d),实验共持续12周。实验期间监测动物的体重、血清肌酐、尿素氮水平的变化。实验结束时,检测各组大鼠肾脏的大体形态,并对肾脏组织进行HE染色病理观察以及透射电镜的检测。结果:模型组动物血清肌酐、尿素氮的水平明显高于对照组,而肌酐清除率明显低于对照组(P<0.05),并且随着实验时间的延长,模型组动物出现进行性的慢性肾功能损伤的改变。模型组动物肾脏的长轴长以及重量都明显高于对照组(P<0.05),并且出现了典型的肾脏损伤的病理学以及超微结构的改变。结论:用腺嘌呤100 mg/(kg·d)给予大鼠灌胃,可以制备出慢性肾功能损伤的模型。

BACKGROUND AND AIM: To make rats' chronic renal function impairment model through adenine induce.MATERIALS AND METHODS: 16 male SD rats were randomly divided into control group and renal function impairment model group.Distilled water was administered orally to rats in the control group and adenine suspension 100 mg/(kg·d) was given to establish the model of renal function impairment.The experiment lasted 12 weeks.Time-dependent changes in serum creatinine(Cr),urea nitrogen(BUN) levels and creatinine clearance rate(Ccr) were monitored.Renal morphological changes were examined.RESULTS: Cr,BUN levels in the model group were higher and Ccr was lower significantly than control group.Renal function impairment was aggravated with extended experimental time.Pathological and ultramicrostructural changes were observed.CONCLUSION: Chronic renal function impairment model in rats could be successfully induced by adenine suspension.