GRP78核酸疫苗在非小细胞肺癌模型中的抗肿瘤作用研究

Anti-tumor activity of GRP78 DNA vaccine on non-small cell hmg carcinoma model

目的:研究GRP78核酸疫苗对非小细胞肺癌的预防作用及生存期影响。方法:将携带GRP78基因的真核表达载体肌肉内注射免疫C57BL/6小鼠,完成3次免疫后,接种小鼠非小细胞肺癌细胞(Tc-1),以此来观察该疫苗对非小细胞肺癌的预防作用及生存期影响。结果:免疫后的治疗组肿瘤生长体积小于对照组,平均生存期比对照组延长了25天,免疫细胞分泌细胞因子肿瘤坏死因子(TNF-α)、γ干扰素(IFN-γ)的水平和对靶细胞的杀伤率明显高于对照组。结论:GRP78核酸疫苗能够提高机体免疫细胞对非小细胞肺癌的杀伤能力,延缓肿瘤生长,延长生存期。

Objective:To study the effect of anti-tumor activity on prophylaxis and survival period by administedng GRP78 DNA vaccine.Methods:The eukaryotic expression plasmid containing GRP78 gene was injected intramuscularly into hind leg of C57BL/6 female mice.After three vaccinations,they were s.c.inoculated with lethal doses of Tc-1 tumor cells.The tumor volumes were measured and survival times were recorded for each individual mouse after tumor challenge.Results:The tumor volumes of treatment group were less than...