摘要
采用响应面法优化了超声波法提取甘草中总黄酮的条件。在单因素实验基础上,选择提取溶剂中乙醇体积分数、提取时间、超声波功率和液料比为随机因子,进行4因素3水平Box-Behnken中心组合设计,采用响应面法(RSM)分析了4个因素对响应值(总黄酮得率)的影响。实验结果表明,超声波法提取甘草中总黄酮的最优条件如下:乙醇体积分数为70.5%,超声波时间为20 min,超声波功率为328 W,液料比(mL:g)为20.8:1。在最优的条件下进行了9次验证实验,总黄酮的平均得率为4.31%,与理论值4.21%的相对误差为2.4%,理论值与实验值相吻合,说明该优化方法可行。
Response surface analysis methodology(RSM)was applied to optimize the ultrasonic- assisted extraction conditions of general flavonoids from glycyrrhiza uralensis.Based on single-factor experiments,four-factors-three-levels Box-behnken central composition experiments design were applied.The concentrations of ethanol in solvent,extraction time,ultrasonic power and ratio of liquid to solid were chosen as causal factors.RSM was employed to study the effect of these factors on the yield of general flavonoids fro...
出处
《吉林大学学报（工学版）》
EI
CAS
CSCD
北大核心
2008年第S2期293-298,共6页
Journal of Jilin University:Engineering and Technology Edition
基金
吉林大学生物实验教学示范中心创新基金二类项目(ZXCX 2006011).
关键词
生物化学工程
甘草
总黄酮
超声波提取法
响应面优化法
biochemical engineering
glycyrrhiza uralensis
general flavones
ultrasound assisted extraction
response surface analysis methodology
