石斛属植物的RAPD指纹图谱聚类分析

Fingerprinting cluter analysis of plants of dendrobium by RAPD

目的建立石斛属植物RAPD指纹图谱,探讨RAPD分子标记技术在石斛属种类鉴别上的应用。方法用RAPD方法构建石斛属内7种石斛及一种石仙桃植物RAPD指纹图谱,对其扩增条带进行分析。结果共扩增出53条带,分布在0.25～1.3kb之间,其中39个条带有遗传多样性,约占总数的73.6℅。经聚类分析不同石斛间的遗传距离分布在0.04762～0.68421,平均遗传距离为0.44380,而石仙桃与石斛属植物的平均遗传距离为0.71734。结论石斛属植物的遗传多态性丰富,与种外植物遗传距离较大,RAPD技术可作为石斛属植物鉴别的有效方法。

Objective To Set up the fingerprinting of species of Dendrobium and discuss the identification of Dendrobium by random amplified polymorphic DNA(RAPD).Methods The RAPD fringerprinting of Dendrobium and its adulterant specise were set up,DNA fragments of RAPD fringerprinting were analysised.Results Eight valid primers produced 53 DNA fragments ranging between 0.25kb and 1.3kb,39 of these fragments were polymorphic bands and accounted for 73.6℅ ;the genetic distance of the twelve Dendrobium spcies varied from...