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EGCG对过氧化氢所致神经元过氧化损伤的保护作用 被引量：11

Protective effect of tea polyphenol (-)-epigallocatechin gallate on peroxidation-injured neurons acutely exposed to hydrogen peroxide
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摘要 目的研究表没食子儿茶素没食子酸酚(EGCG)对过氧化氢(H2O2)所致神经元过氧化损伤的保护作用。方法分离胚胎18d大鼠大脑皮质神经元,原代培养2d后分为4组:①正常细胞对照组;②药物对照组(正常细胞内加EGCG);③氧化损伤组(正常细胞内加H2O2);④损伤后药物治疗组(氧化损伤30min后加入EGCG)。各组细胞再培养36h后,进行神经元形态观察、细胞活性MTT分析及细胞中丙二醛(MDA)含量的检测。结果氧化损伤组神经元多崩解成碎片,突起不完整,细胞活性显著降低,MDA含量显著升高。而在损伤后药物治疗组,神经元胞体立体感较强,多数突起完整。细胞活性显著增高,而MDA含量则显著下降。结论EGCG可抑制羟基自由基的产生。 Objective To investigate the effect of tea polyphenol (-) -epigallocatechin gallate (EGCG)on peroxidation-injured neurons acutely exposed to hydrogen peroxide (H2O2). Methods Cortical neurons from embryonic 18 day rats were primarily cultured for 2 days, and divided into 4 groups: ①Normal neuron control group, ②Normal neuron with EGCG group, ③H2O2 -injured neuron group (normal neurons with the treatment of H2O2), and ④EGCG-treatment group (Injured neurons treated with EGCG 30 minutes after H2O2 administrati...
作者 焦西英 杨浩 钱新宏 武明媚 胡沛臻 游思维
机构地区 第四军医大学全军神经科学研究所 第四军医大学西京医院儿科 第四军医大学基础部病理学教研室
出处 《解剖学研究》 CAS 2008年第6期401-403,483+404,共5页 Anatomy Research
基金 陕西省自然科学基金(2006C29) 陕西省攻关计划课题(2007K15-01(2)) 国家自然科学基金(30571998)
关键词 表没食子儿茶素没食子酸酚 过氧化氢 神经元 细胞培养 Tea polyphenol （-） -epigallocatechin gallate Hydrogen peroxide Neuron Cell culture
分类号 R285.5 [医药卫生—中药学]
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参考文献22

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共引文献6

同被引文献140

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引证文献11

二级引证文献33

解剖学研究
2008年 第6期

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