夏至草醇提物对大鼠急性微循环障碍的影响

Effects of ethanol extract from Mrrubium Incisum(夏至草醇提物) on acute microcirculatory disturbance in rats

目的观察夏至草醇提物对高分子右旋糖酐(Dextran 500)致急性微循环障碍(AMD)大鼠肠系膜血液及淋巴微循环的影响。方法20只雄性Wistar大鼠被随机分为夏至草组和模型组,每组10只。左颈静脉注射Dextran 500(10 ml/kg)复制AMD模型。6 min后夏至草组自颈静脉缓慢推注夏至草醇提物(6 g/kg),模型组给予等量生理盐水。应用活体显微电视录像系统观察大鼠肠系膜血液及淋巴微循环的变化。结果与实验前比较,模型组大鼠表现为肠系膜微血管口径缩窄、微血流流速变慢,伴有红细胞聚集,微血流流态积分值显著增高;肠系膜微淋巴管(ML)自主收缩频率、收缩活性指数(Index)、总收缩活性指数(Index)、淋巴动力学指数(LD-Index)均显著降低(P均<0.05)。注入夏至草醇提物后,大鼠肠系膜微血管口径较模型组扩张,血流加速,微血流流态积分明显降低,ML自主收缩频率、Index、Index、LD-Index显著升高(P均<0.05)。结论夏至草醇提物能明显减轻Dextran 500所致AMD大鼠肠系膜血液及淋巴微循环障碍。

Objective To observe the effects of ethanol extract from Mrrubium Incisum(EEMI,夏至草醇提物) on mesenteric blood and lymphatic microcirculation in rats with acute microcirculatory disturbance(AMD) induced by Dextran 500.Methods Twenty male Wistar rats were randomly divided into two groups: EEMI group(n=10) and model group(n=10).The AMD model was established by intravenously administrating Dextran 500(10 ml/kg) via left carotid vein.Six minutes later,EEMI(6 g/kg) was slowly infused into the carotid vein in EEMI gr...