白花蛇舌草注射液抗肿瘤和免疫调节作用实验研究

Experimental Study on Antitumor Activities and Immunity Regulation of Hedyotis Diffusa Willd Injection

目的研究白花蛇舌草注射液抗肿瘤及对荷瘤小鼠的免疫调节作用。方法采用MTT比色法,检测白花蛇舌草注射液对肿瘤细胞株增殖的抑制作用,计算抑制率。测定荷瘤小鼠脾脏NK细胞活性、腹腔巨噬细胞吞噬功能、脾细胞增殖的影响、血清凝集素活性和脾脏IL-2活性5项指标。结果白花蛇舌草注射液对A549、SGC-7901、HEP-G2、Hela的增殖,剂量在100～20μg/mL范围时,均有不同程度的抑制作用。白花蛇舌草注射液能明显增强荷瘤小鼠脾NK细胞杀伤活性,增强处于IL-2活性严重低下状态下荷瘤小鼠的IL-2活性,增强荷瘤小鼠脾细胞增殖,增强荷瘤小鼠腹腔巨噬细胞吞噬功能,提高荷瘤小鼠体液免疫血清中抗羊红细胞凝集素值。结论白花蛇舌草注射液具有体外抗肿瘤及提高荷瘤小鼠免疫功能的作用。

Objective To study the antitumor activities and immunity regulation of Hedyotis diffusa Willd injection. Methods Inhibitory effects of Hedyotis diffusa Willd injection on cell proliferation was detected with MTT method. Then the inhibitive rate was calculated. The immunomdulatory effects of Hedyotis diffusa Willd injection were investigated. Results Hedyotis diffusa Willd injection inhibited the proliferation of A549, SGC-7901, HEP-G2, Helacells in the dose of 100～20 μg/mL, and enhance the immunity regulati...