高效液相色谱法测定饲料中维吉尼亚霉素的含量

Determination of Verginamycin in Feed Using HPLC

研究并建立了饲料中维吉尼亚霉素含量的高效液相色谱测定方法。样品用乙酸乙酯提取,经硅胶柱和HLB固相萃取柱净化,在210 nm波长下用紫外或二极管矩阵检测器检测。维吉尼亚霉素浓度在2~500μg/mL范围内与峰面积呈良好的线性关系,回收率为86.3%~93.3%,变异系数为3.33%~5.37%,检出限和定量限分别为0.5 mg/kg和2 mg/kg。实验结果表明该法准确度高、重现性好、结果可靠。

A HPLC method to determin the contents of verginamycin in feed was established.The sample was extracted with ethylacetate,purified by silica cartridge and HLB cartridge,and then detected with UV detector or DAD at 210 nm.The concentration of verginamycin and peak area was linear correlation between 2～500 μg/mL,the reclaim ratio was 86.3%～93.3% and RSD was 3.33%～5.37% The detection limit was 0.5 mg/kg and the limit of quantification was 2 mg/kg.The results indicated that the constructed method was accurate,r...