应用两种PCR方法检测饲料中牛、羊源性成分

Detection of Dovine and Sheep Derived Materials in Feed by Two PCR Methods

对普通PCR和实时(realtime)PCR两种方法在饲料中牛、羊源性成分检测中的应用进行了比较。分别设计并合成相应的引物和探针,对饲料中的牛、羊源性成分进行检测。结果表明,用普通PCR最低可以从饲料中检出0.1%的牛、羊源性成分,realtime PCR最低可以从饲料中检出0.01%的牛、羊源性成分。

Two polymerase chain reaction methods for the detection of bovine and sheep derived materials in feed were compared.Bovine and sheep derived materials were detected by correponding primers and probes.The result showed that 0.1%bovine and sheep derived materials could be detected by PCR method,and 0.01% bovine and sheep derived materials could be detected by realtime PCR method.