非小细胞肺癌患者血清中ADAM8和CEA的表达及意义

Expression and clinical significance of ADAM8 and CEA in serum of patients with non-small cell lung cancer

背景与目的肺癌是目前世界上最常见的恶性肿瘤,死亡率在各类肿瘤中居首位,本文拟研究ADAM8和CEA在非小细胞肺癌患者血清中的表达规律,探讨ADAM8和CEA对非小细胞肺癌的诊断价值。方法应用酶联免疫吸附试验方法检测62例非小细胞肺癌患者、27例良性肺病患者和32例健康体检者血清中的ADAM8蛋白表达水平,同时用放射免疫分析法检测以上所有研究对象血清中CEA的蛋白表达水平。结果血清ADAM8、CEA水平在NSCLC组均高于良性肺病组和正常对照组,差异有统计学意义(P<0.01),良性肺病组中这两种标志物表达水平比正常对照组略高,但差异无统计学意义(P>0.05);ADAM8在腺癌患者中表达水平与鳞癌相比差异无统计学意义(P>0.05),而CEA在腺癌患者中表达水平比鳞癌高,差异有统计学意义(P<0.05);Ⅲ-Ⅳ期NSCLC患者血清中ADAM8和CEA的表达水平均高于Ⅰ-Ⅱ期患者,差异均有统计学意义(P<0.01);伴有淋巴结转移NSCLC患者血清中ADAM8和CEA的表达水平均高于不伴有淋巴结转移者,差异均具有统计学意义(P<0.01);单项检测ADAM8、CEA对NSCLC的敏感性分别为77.4%、71.0%,特异性分别为90.6%、84.4%,联合检测敏感性提高到91.9%,但特异性下降到75.0%。结论ADAM8在非小细胞肺癌患者血清中的过度表达表明其与肺癌的发展有着密切的关系,联合检测ADAM8和CEA对非小细胞肺癌的诊断有一定的辅助价值。

Background and objective Lung cancer is one of the most common cancers in the world,whose mortality is at the first place in all malignant tumors.This study is to investigate the diagnostic value of ADAM8 and CEA in serum of non-small cell lung cancer patients.Methods The serum levels of ADAM8 were assayed by ELISA in 62 NSCLC patients,27 benign palmonary lesions and 32 healthy subjects as control.The serum level of CEA were assayed by radioimmunoassay.Results The serum level of ADAM8 and CEA in group of NS...