MTA1基因在非小细胞肺癌中的表达及其意义

Expression and clinical significance of MTA1 in non-small cell lung cancer

背景与目的转移相关基因(metastasis-associated gene 1,MTA1)是近年来研究较深入的肿瘤浸润转移基因,在多种肿瘤细胞系中表达,与肿瘤的侵袭和转移密切相关。本研究目的是探讨MTA1基因的表达与非小细胞肺癌的浸润、转移关系。方法建立测定MTA1mRNA表达的巢式逆转录聚合酶链反应(RT-PCR)最适条件,在半定量水平测定42例非小细胞肺癌标本癌组织、癌旁组织、正常组织和淋巴结及20例肺良性疾病组织MTA1mRNA表达的相对水平,分析其与临床和组织病理学特征的关系。结果MTA1mRNA在非小细胞肺癌组织(1.50±0.26)及转移淋巴结(1.88±0.35)中的平均表达水平高于正常组织(1.02±0.17)和良性疾病组织(0.90±0.15)(P<0.01),癌组织中MTA1的表达(1.50±0.26)高于癌旁组织(1.09±0.16)(P<0.01),淋巴结转移组MTA1的表达(1.88±0.35)高于无淋巴结转移组(1.40±0.36)(P<0.01),非小细胞肺组织中MTA1mRNA高表达率与临床分期、T分期和N分期呈正相关关系。42例非小细胞肺癌组织中,19例MTA1mRNA高表达,占45.2%;19例有转移的淋巴结中,16例高表达占84.2%。MTA1在癌组织中的表达与患者年龄、性别、肿瘤类型无关(P>0.05)。结论MTA1mRNA过度表达与非小细胞肺癌浸润和转移密切相关。MTA1mRNA的过度表达可能是评价非小细胞肺癌的恶性程度、转移的潜在指标。

Background and objective Metastasis-associated gene 1(MTA1) has been studied deeply recently as a tumor infiltration and metastasis gene.It was expressed in many tumor cell line and was correlated with tumor infiltration and metastasis.The aim of this study is to investigate the relationship between the expression of MTA1 and invasion and metastasis of non-small cell lung cancer(NSCLC).Methods Optimal conditions of nested reverse transcription polymerase chain reaction(RT-PCR) were found out;then the expres...