流动剪切应力下体外培养人体滋养层细胞的迁移和β1整合素表达

Migration and β1 integrin expression of cultured human trophoblast cells exposed to flow-induced shear stress

目的在人胎盘发育过程中,滋养层细胞群体侵入子宫内膜的毛细血管,并沿母体脉管系统逆流迁移,最终停留在母体螺旋动脉对其结构进行重构。本文目的是验证我们的推测:血液流动剪切力可以调控人体滋养层细胞黏附分子β1整合素的表达,从而影响滋养层细胞的迁移。方法体外培养人体胚胎滋养层细胞,测量流动剪切力下滋养层细胞的迁移和β1整合素表达,所加载的剪切力大小为0,75,150和300Pa4个水平,时间24h。结果在剪切力作用下,滋养层细胞迁移活性随剪切力的增高而显著增加,在流动剪切力方向存在优势性的迁移。同时,剪切力还增加了β1整合素的表达。结论流动剪切力的参与调节了滋养层细胞β1整合素的表达,从而可影响滋养层细胞的迁移。

Objective During fetal development, trophoblast cells enter endometrial capillaries, migrate against blood stream within the uterine vasculature, and eventually reside within spiral arteries of the human uterus.In this paper, the hypothesis was tested that by regulating the expression of β1 integrin in trophoblast cells, flow-induced shear stress affects the motility of trophoblast cells.Method Early gestation human trophoblast cells were cultured in glass piece and then exposed to steady shear stresses of ...