喉鳞癌中p73、p63的表达及其临床意义

Expression and biological significance of p73 and p63 in laryngeal squamous cell carcinoma

目的:探讨p73、p63在喉鳞癌(LSCC)组织中的表达及与喉鳞癌发生、发展的关系和生物学意义。方法:应用原位分子杂交方法,检测p73mRNA、p63mRNA在47例喉鳞癌组织、癌旁组织及14例正常喉组织中的表达。结果:p73在正常喉组织中不表达;在喉癌组织、癌旁组织中的阳性表达率分别为57.4%(27/47)、29.8%(14/47),分别与正常喉组织比较,差异有统计学意义(P<0.05);组织分化程度越低、TNM分期越晚、淋巴结转移率越高,则p73 mRNA表达越强(均P<0.05)。p63在喉鳞癌、癌旁组织中的阳性表达率为91.5%(43/47)、93.6%(44/47)、分别与正常喉组织(57.1%)比较,差异有统计学意义(P<0.05);p63与喉癌临床病理特征无关。结论:p73表达有助于判断喉鳞癌的生物学行为和预后。p63对LSCC生物学行为的影响较小。

Objective:To investigate the expression of p73 and p63 and their biological significance in laryngeal squamous cell carcinomas(LSCC).Methods: The expression of p73 and p63 in 47 cases of tumor tissues、adjacent and normal tissues were detected using in situ hybridization.Results: Percentage of p73 expression was 57.4%(27/47),29.8%(14/47)in tumor tissues and adjacent tissues respectively.In contrast,normal laryngeal tissues did not express p73.Overexpression of p73 was strongly associated with tumor grade,TNM...