植入电极记录豚鼠图形视觉诱发电位

Recording of pattern visual evoked potential in guinea pigs with implanted electrodes

目的探讨用植入电极方式记录豚鼠图形视觉诱发电位(pattern visual evoked potential,PVEP)的方法。方法豚鼠麻醉后,暴露颅骨表面,在前囟(Bregma)前6mm、后10mm及矢状缝正中处钻孔,均植入长5mm、直径为1.2mm的不锈钢螺钉,分别作为参考电极、记录电极和地极。3d后,将清醒状态下的豚鼠固定于自制多功能头身一体化固定台面上,银-氯化银电极连接于豚鼠体表的不锈钢螺钉上,采用RETI-port系统(Roland Consult,德国)分别记录不同空间频率和不同颜色下的图形视觉诱发电位。结果植入电极后,在无出血感染条件下,实验豚鼠生存时间与正常豚鼠没有明显区别。在其清醒状态下可以记录到豚鼠的PVEP波形,呈NPN形状。空间频率对PVEP波形有明显的影响:随着空间频率的增加,N1、P1波的潜伏期都有不同程度的延长,当空间频率大于0.5cpd时,潜伏期延长明显;随着空间频率的增加,振幅降低。在不同对比度(97%、60%、30%)下,P1波的振幅随着对比度的增加而提高,且任意两个对比度之间P波振幅的差异均有统计学意义(P<0.05)。

Objective To establish a new method for recording pattern visual evoked potentials(PVEP)in guinea pigs.Methods After anesthesia,the guinea pig s scalp surface was exposed and two stainless steel screws(5 mm long and 1.2 mm diameter)were implanted 6 mm in front of and 10 mm behind the Bregma,as a reference electrode and recording electrode,respectively.A third stainless steel screw was implanted in the middle as a ground elec-trode.Three days later,the awake guinea pig was firmly placed in a multifunctional ...