釉原蛋白对牙髓硬组织形成的诱导作用

Induction of amelogenin in formation of hard tissues in dental pulp

目的:探讨釉原蛋白对牙髓硬组织形成的诱导作用,为釉原蛋白诱导硬组织形成功能的研究提供实验依据。方法:Wistar雄性大鼠16只,随机分为正常对照组和实验组[连续注射环磷酰胺(CP)14d],采用组织学和免疫组织化学的方法,观察切牙成牙本质细胞及牙髓组织发生的各种变化及与釉原蛋白的关系。结果:实验组注射CP后切牙形成端处于细胞增殖期和分化期的成牙本质细胞和牙髓细胞发生了广泛的水肿、变性甚至部分坏死、消失。牙髓损害区唇侧成釉器对应的牙髓侧,可见骨样牙本质样的硬组织形成;成釉器的增殖端附近可见成釉细胞分化,而且在新生硬组织表面分泌很厚的釉基质,经免疫组织化学染色釉原蛋白呈阳性表达。正常对照组无变化。结论:CP抑制成牙本质细胞形成后,釉原蛋白具有诱导牙髓形成硬组织的作用。

Objective To study the induction of amelogenin in formation of hard tissues in the pulp tissues,to provide experimental foundation for research in formation of hard tissues induced with amelogenin.Methods Sixteen male Wistar rats were divided into control group and experimental group.Cells and matrix structures and amelogenin expression in the growing ends of cyclophosphamide(CP)-affected incisors were examined by histological and immunohistochemical methods.Results Experiment group:extensive edematous chan...