摘要
利用不同蔗糖浓度、硼酸浓度、Ca2+浓度、温度对6个不同熟性的大麻品种的花粉进行萌发研究,结果表明:大麻花粉培养以15%蔗糖+80mg/L硼酸的液体培养基时花粉的萌发率最高,花粉管的生长速度则以硼酸浓度为20mg/L时最快,加入Ca2+则抑制花粉萌发,培养温度25℃最好。花粉储存中,常温下,未干燥的大麻花粉生活力只能保持3d(天);干燥、密封后在1—4℃条件下花粉活力最多可保持30d;干燥、密封后在-20℃条件下,储存120d后的花粉还有较高的萌发率(34%)。
The test of pollen germination of six different hemp varieties was conducted by setting different temperatures and concentrations of sucrose, boric acid and Ca2+. The results showed that hemp pollen germination rates reached the highest when being cultured in the liquid medium containing 15% sucrose and 80mg/L boric acid , while the highest growth rate of pollen tube was obtained under the condition of 20mg/L boric acid, and Ca2+ inhibited the germination, the optimal temperature was 25℃. When being stored ...
出处
《中国麻业科学》
2008年第6期305-310,共6页
Plant Fiber Sciences in China
基金
国家科技支撑计划项目(2006BAK0903)
关键词
大麻花粉
萌发条件
萌发率
花粉管
花粉储存
hemp pollen
germination condition
germination rate
pollen tube
pollen storage
