阿托伐他汀对肿瘤坏死因子α诱导的大鼠主动脉平滑肌细胞内皮脂肪酶表达的影响

Effects of Atorvastatin on Tumor Necrosis Factor-α Induced Endothelial Lipase Secreting by Rat Vascular Smooth Muscle Cell

目的观察阿托伐他汀对肿瘤坏死因子α诱导的大鼠主动脉平滑肌细胞内皮脂肪酶表达的影响。方法原代培养的大鼠主动脉平滑肌细胞中先加入50μg/L的肿瘤坏死因子α,1h后加入不同浓度(1×10-7mmol/L、1×10-6mmol/L和1×10-5mmol/L)的阿托伐他汀,继续孵育24h后,收集细胞,采用逆转录聚合酶链反应检测培养的平滑肌细胞中内皮脂肪酶mRNA的表达情况。另外,取阿托伐他汀1×10-5mmol/L浓度,分别在6、12和24h用同样的方法检测培养细胞中内皮脂肪酶mRNA表达情况。结果随阿托伐他汀浓度的增加,肿瘤坏死因子α诱导的平滑肌细胞内皮脂肪酶mRNA表达水平呈下降趋势,与对照组比较差异有显著性(P<0.05),不同浓度组之间比较亦有统计学意义(P<0.05);在1×10-5mmol/L阿托伐他汀作用下,随着作用时间的延长平滑肌细胞内皮脂肪酶mRNA表达水平亦呈下降趋势,与对照组比较有统计学意义(P<0.05),不同时间组之间比较亦有统计学意义(P<0.05)。结论随着阿托伐他汀浓度的增加与作用时间的延长,肿瘤坏死因子α诱导的平滑肌细胞内皮脂肪酶mRNA的表达均呈下降趋势。

Aim To investigate the effects of atorvastatin on TNF-α induced endothelial lipase secreting by rat vascular smooth muscle cells. Methods The rat vascular smooth muscle cells were obtained from thoracic aortas and cultured by tissue explant method. Rat vascular smooth muscle cells were first incubated in serum-free medium with 50 μg/Ltumor necrotic factor-alpha; 1 hour later,atorvastatins with different concentration(1×10-7 mmol/L ,1×10-6mmol /L,1×10-5 mmol/L)were added to the medium. 24 hours later, cells ...