三羊益心康胶囊对犬急性心肌缺血损伤保护作用的实验研究

Effect of Sanyangyixinkang Capsule on Acute Myocardial Ischemia in Dogs

目的:观察三羊益心康胶囊的抗心肌缺血作用。方法:采用结扎犬冠状动脉前降支法制备急性心肌梗死模型,利用心外膜电图标测法计算心肌缺血程度,硝基四氮唑蓝(NBT)组织化学染色法测定心肌梗死面积,血液检测天门冬氨酸氨基转换酶(AST)、肌酸磷酸激酶(CPK)、乳酸脱氢酶(LDH),在电镜下观察心肌病理组织改变,观察了三羊益心康胶囊对心肌缺血损伤保护作用。结果:三羊益心康胶囊可明显降低心肌缺血程度(∑-ST),减少心肌缺血范围(N-ST),缩小心肌梗死面积(MIS),并能显著降低血清AST和CPK含量,明显减轻心肌细胞损伤程度。结论:该药对犬急性心肌缺血具有明显的保护作用。

Objective:To investigate the effect of Sanyangyixinkang Capsule on acute myocardial ischemia in dog.Methods:Acute myocardial infarction model in dog was established by ligating anterior descending coronary artery,using epicardial electrogram mapping to monitor the level of myocardial ischemia,using NBT histochemical stain to determine myocardial infarction size;using biochemical methods to determine AST,CPK,LDH;and using electron microscopy method to observe the morphologic changes of myocardial ischemia.Re...