生血合剂干预下免疫介导再障小鼠骨髓核因子κB表达变化

Changes for NF-κB in Bone Marrow of Mice Model for Immune-mediated Aplastic Anemia Controled by Shengxue Mixture

目的:进一步探讨获得性再生障碍性贫血(AA)发病机制及健脾补肾活血方生血合剂治疗AA的疗效机制。方法:以环孢素为对照,免疫介导AA小鼠为研究对象,采用定量PCR(Q-PCR)、免疫组织化学等方法分析生血合剂干预前后AA小鼠骨髓核因子κB(NF-κB)mRNA水平、蛋白表达及活性变化。结果:AA小鼠骨髓单个核细胞NF-κB mRNA水平未见明显改变,与正常对照比无显著差异(P>0.05);骨髓细胞NF-κB/p65蛋白表达较正常组增加,p-IκBα表达明显下降,生血合剂干预后NF-κB/p65蛋白表达减少,而p-IκBα表达明显提高;与之相比,环孢素作用后NF-κB/p65蛋白及p-IκBα均急剧下降。结论:NF-κB蛋白表达异常及活性改变可能参与了免疫介导AA小鼠模型的形成;生血合剂可能通过调节NF-κB的表达及活性发挥改善免疫介导AA小鼠造血功能的作用。

Objective:To explore further the pathogenesis of acquired aplastic anemia(AA) and the curative effect mechanism of Shengxue Mixture(SXM),witch is a Chinese materia medica compound with the function of reinforcing the spleen and nourishing the kidney and promoting blood circulation.Methods: Cyclosporin A as a control,mRNA and protein level and activity of NF-κB in bone marrow of mice model for immune-mediated aplastic anemia(AA) were detected with Q-PCR and immunohistochemistry technique before and after giv...