腺病毒携带凋亡素基因导入对小鼠胃癌的影响

Effect of adenovirus vector carrying apoptin gene on mice gastric cancer

目的研究携带凋亡素(VP3)基因的腺病毒对小鼠胃癌的作用。方法将小鼠前胃癌细胞接种于昆明小鼠腋部皮下成瘤,实验组瘤内注射AdCMV-VP3,两对照组分别注射等量的AdCMV和PBS,每隔1d测量各组肿瘤体积,治疗15d后,比较3组肿瘤体积的变化并计算抑瘤率。将小鼠脱颈处死,用TUNEL法检测肿瘤组织凋亡情况,用RT-PCR检测VP3基因的表达。将荷瘤小鼠按肿瘤直径分组,实验组均注射等量AdCMV-VP3,对照组均注射等量PBS,治疗l5d后,计算抑瘤率。再将同直径肿瘤的荷瘤小鼠按AdCMV-VP3不同注射量分组,对照组注射等量PBS,治疗l5d后,计算抑瘤率。结果实验组的抑瘤率为88.4%,AdCMV对照组的抑瘤率为6.5%,3组肿瘤体积变化的差异有统计学意义(P<0.05)。癌组织中细胞死亡方式以凋亡为主,VP3基因仅在实验组表达。不同肿瘤直径抑瘤率分别是88.4%、73.5%、68.9%,不同剂量抑瘤率分别是63.5%、74.4%、80.5%、88.4%。结论腺病毒携带VP3基因导入对小鼠实验性胃癌有较好的抑癌作用。肿瘤体积越小,腺病毒用量越大,疗效越好。

【Objective】 To investigate the effect of adenovirus vector carrying the VP3 gene on mouse gastric cancer.【Methods】The mice gastric cancer model was established by inoculating mouse fore-stomach carcinoma (MFC) at the armpit.The experimental group was intratumorally injected with AdCMV-VP3 and two control groups were intratumorally injected with AdCMV and PBS.The volume of tumor was observed each two days.The tumor volume of the three groups were compared with each other and the inhibition rate was calculate...