摘要
目的制备荧光量子点标记的叶酸偶联白蛋白纳米粒,并研究该纳米粒对人结肠癌HT-29细胞的靶向性。方法先通过缩合剂将量子点与白蛋白纳米粒相结合,再利用叶酸活性酯在碱性条件下与白蛋白表面上的氨基反应,制得叶酸修饰的白蛋白/量子点纳米粒,通过量子点荧光标记的方法对结肠癌HT-29肿瘤细胞摄取Folate-HAS/QDs纳米粒进行相关研究。结果成功制备了具有良好粒径分布和荧光性能的Fo-late-HAS/QDs纳米粒,并证实该纳米粒对HT-29细胞有较强的靶向性。结论通过量子点作为荧光标记物,简单直观的证实了叶酸修饰的白蛋白/量子点纳米粒能通过细胞膜上的叶酸受体介导内吞入细胞,可显著靶向于叶酸受体丰富的肿瘤细胞。
Objective To prepare folate-conjugated albumin nanoparticles with quantum dots labeling and study its targeting effect in HT-29 cells. MethodsAlbumin nanoparticles were coupled with quantum dots and the activated folic acid was conjuated to the surface of HAS/QDs NPs in alkalinity. Study on the uptake of Folate-HAS/QDs NPs by HT-29 cells by the methods of fluorescent labeling of QDs. ResultsFolate-HAS/QDs NPs were successfully prepared with good morphology and fluorescent properties,and we confirmed these n...
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第23期3418-3420,共3页
China Journal of Modern Medicine
关键词
量子点
叶酸受体
白蛋白纳米粒
靶向性
quantum dots
folate receptor
albumin nanoparticles
targeting
