雷帕霉素药物涂层支架对家猪冠状动脉NF-κB影响的研究

Expression of NF-κB on susscrota domestica coronary artery after rapamycin eluting stent implanting

目的探讨核因子-κB(nuclear factor-kappa B,NF-κB)mRNA和蛋白是否参予介入后支架内再狭窄(in-stent restennosis,ISR)的发生发展以及雷帕霉素药物涂层支架(rapamycin eluting stent,RES)是否影响NF-κB mRNA和蛋白表达。方法将裸金属支架(bare metal stent,BMS)和RES按支架∶动脉比率(直径1.1～1.2∶1.0)随机植入猪冠状动脉前降支或回旋支,1个月后行冠状动脉造影并取材,冠脉定量造影术(quantity of coronary artery,QCA)分析平均血管直径、晚期血管丢失和径向狭窄,血管组织标本嗜伊红染色后测定支架血管段冠状动脉内膜最大厚度、内膜面积和支架段内血管腔面积,Rt-PCR检测NF-κB mRNA表达和Western blotting检测NF-κB蛋白表达。结果①与BMS组相比较,RES组平均血管直径显著改善(P<0.05),而晚期血管丢失和径向狭窄均显著减少(均P<0.05)。②BMS组和RES组均可见内膜完全覆盖支架,BMS组支架段内内膜最大厚度和内膜面积均显著大于RES组(均P<0.05)、血管腔面积显著小于RES组(P<0.05)。③正常血管段可见NF-κB mRNA和蛋白微量表达,与此相比,BMS组和RES组血管段NF-κBmRNA和蛋白表达均显著增强,不过,RES组血管段NF-κB mRNA和蛋白显著低于BMS组血管段。④内膜面积与NF-κB mRNA(r=0.349,P=0.001)和蛋白(r=0.417,P=0.001)呈显著正相关。结论NF-κB可能介导ISR进程,BMS可能通过促进NF-κB导致ISR而RES则可能通过抑制NF-κB减轻ISR。

ObjectiveTo elucidate whether nuclear factor kappa B(NF-κB) mRNA and its protein take part in the proceed of in-stent restennosis(ISR) and whether rapamycin eluting stent(RES) influences on their expression after stent embedding coronary artery of susscrota domestica. Methods According to the ratio of stent vs artery(diameter 1.1～1.2∶1.0) ,both bare metal stent(BMS) and RES were implanted in the anterior descending coronary or circumflex branch coronary. After 1 month,coronary arteriongraphy was finished to...