前列腺素D2受体基因T-549 C位点多态性和哮喘相关性研究

Correlation between PTGDR-549 T/C polymorphism and susceptibility of asthma

目的探讨前列腺素D2受体(PTGDR)基因T-549C位点和哮喘易感性的相互关系。方法对照组和哮喘组各100例。取外周血提取DNA,用RFLP-PCR方法检测前列腺素D2受体基因T-549C位点的分布。结果对照组和哮喘组等位基因T、C分布差异有显著性,χ2=5.78,P<0.01,C等位基因和哮喘相关OR(C/T)=2.07,P<0.01。两组基因型CC、TC与TT相比,频率的分布差异亦有显著意义,OR(CC/TT)=4.83,P<0.01;OR(TC/TT)=2.55,P<0.01。结论CC型纯合子患哮喘的危险性最高,PTGDR是哮喘的易感基因。

ObjectiveTo investigate the correlation between the polymorphism at-549 locus in PTGDR gene and the susceptibility of asthma. Methods100 asthma subjects and 100 control subjects were chosen. DNA was isolated from the blood. Polymerase chain reaction/restriction fragment length polymorphism(PCR/RFLP) techniques were used to determine-549 locus polymorphism. ResultsThe distribution of T and C alleles of-549 are signifi-cantly different between the two group,(χ2=5.78,P <0.01) ,C allele is associated with as...