葛根素对大鼠视网膜缺血再灌注损伤中细胞超微结构的影响

Effect of puerarin on ultrastructual of retina which injuried by ischemia-reperfusion

目的探讨葛根素对大鼠视网膜缺血再灌注损伤超微结构的影响。方法建立大鼠视网膜缺血再灌注模型,并分缺血再灌注组和缺血再灌注+葛根素治疗组。每组按再灌注时间的不同分为缺血再灌注1、6、12、24、48和72h组。每组均行细胞超微结构和ERG检测。结果在缺血再灌注组,各组视网膜细胞在再灌注1h后开始改变,24h后损害最严重,以后逐渐有所恢复。在缺血再灌注+葛根素组,视网膜细胞结构也在6h后开始明显改变,24h后受损最重,但明显好于缺血再灌注未处理组。同时,缺血再灌注+葛根素组各时期ERGa、b波波幅相对恢复率高于另两组。结论葛根素对大鼠视网膜缺血再灌注损伤的细胞超微结构有保护作用。

[Objective] To study The effect of puerarin on ultrastructual of retina which injuried by ischemia-reperfusion.[Method] The model of retinal ischemia-reperfusion was emploied by ligationed the left side of artery,the rats was devided into ischemia-reperfusion group and ischemia-reperfusion and puerarin group,each group including 1 h,6 h,12 h,24 h,48 h and 72 h.the ERG were examined in different stages.At the same time,the ultrastructure of retina were examined in different stages.[Result] In ischemia-reperf...