重型再生障碍性贫血患者血清诱导32D细胞凋亡并下调STAT5基因表达

Sera from patients with severe aplastic anemia induce apoptosis and down-regulate expression of STAT5 gene in 32D cells

目的:探讨重型再生障碍性贫血(SAA)患者血清诱导32D细胞凋亡过程中STAT5基因表达情况。方法:用SAA患者和正常对照者血清分别孵育小鼠32D细胞24h后检测细胞存活率、细胞凋亡率和细胞内STAT5a/b基因的mRNA表达水平。结果:与正常对照组(n=8)相比,SAA组(n=9)细胞存活率降低,细胞凋亡率则高于正常对照组,且差异均有显著性。SAA组细胞STAT5a/b基因mRNA的表达显著低于正常对照组。结论:SAA患者血清在体外作用24h后可诱导32D细胞凋亡并下调STAT5a/b基因mRNA的表达,SAA患者血清可能通过下调STAT5a/b的表达诱导32D细胞凋亡。

Objective:To investigate the expression of STAT5 gene in apoptosis of the myeloid progenitor cell line 32D induced by sera from patients with severe aplastic anemia(SAA).Methods:The 32D cells were subjected to apoptosis analysis using flow cytometry after treated with normal sera or sera from patients with SAA for 24 hours respectively.Cell counting and trypan blue staining were performed to assess cell viability.The expression of STAT5a/b gene was detected by RT-PCR.Results:The 32D cells of the SAA group(n...