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定量测定45例血小板膜糖蛋白及其在急性脑梗死中的意义 被引量：2

Quantitative analysis of platelet membrane glycoprotein and its clinical significance in acute cerebral infarction
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摘要 目的:探讨流式细胞术定量测定血小板膜糖蛋白在急性脑梗死中的临床意义。方法:应用流式细胞仪测定静息状态和TRAP激活的血小板所结合单抗的分子数。结果:急性脑梗死患者血小板静息状态下GPⅢa和GPⅡb/Ⅲa密度[分别为(76.8±14.1)×103/血小板、(73.9±10.5)×103/血小板]高于健康对照组[分别为(55.6±5.8)×103/血小板、(50.5±4.1)×103/血小板,P<0.05],TRAP活化后患者血小板GPⅢa和GPⅡb/Ⅲa密度[分别为(102.6±16.4)×103/血小板、(108.5±16.7)×103/血小板]亦明显高于对照组[分别为(69.7±18.3)×103/血小板、(61.2±5.7)×103/血小板,P<0.01];患者血小板静息状态下,GMP-140表达与对照组比较没有显著性差异(P>0.05),而TRAP激活后患者血小板GMP-140表达[(1.21±0.41)×103/血小板]明显高于对照组[(0.87±0.26)×103/血小板,P<0.05];患者血小板静息和活化状态GPⅠb表达与对照组比较没有显著性差异(P>0.05)。结论:急性脑梗死患者血小板呈较为活化状态,血小板膜糖蛋白测定对病情的分析和疗效的判断有重要的临床参考价值。 Objective:To explore the flow cytometric quantitative analysis of platelet membrane glycoprotein and its clinical significance in acute cerebral infarction.Methods:The molecule numbers of platelet GPⅠb,GPⅢa,GPⅡb/Ⅲa and GMP-140 on resting platelets and platelets activated by TRAP were detected by Flow cytometry.Results:Whether on resting platelets or platelets activated by TRAP,the surface densitits of GPⅢa and GPⅡb/Ⅲa in patients with cute cerebral infarction were significantly higher than those in healthy ...
作者 江雁 穆红 唐志琴 黄繁嫱
机构地区 天津市第一中心医院检验中心
出处 《陕西医学杂志》 CAS 北大核心 2008年第12期1672-1674,共3页 Shaanxi Medical Journal
关键词 脑梗塞/诊断 血小板膜糖蛋白类 流式细胞术 Brain infarction/diagnosis Platelet membrane glycoproteins Flow cytometry
分类号 R743.3 [医药卫生—神经病学与精神病学]
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参考文献5

  • 1[1]Shimizu M,Kohara S,Yamamoto M,et al.Significant relationship between platelet activation and intra-media thickness of the carotid artery in patients with ischemic cerebrovascular disease.Thromb Res,2006,117 (6):647-652.
  • 2[2]Shimizu M,Kohara S,Yamamoto M,et al.Link between platelet activity and outcomes after an ischemic stroke.Cerebrovasc Dis,2005,20(2):120-128.
  • 3[3]Michelson AD.Flow cytometry:A clinical test of platelet function.Blood,1996,87:4925-4936.
  • 4[4]Nurden AT,Cazes E,Bihour C,et al.Confirmation that GP Ⅰ b-Ⅸ complexes have reduced surface distribution on platelet activated by thrombin and TRAP-14-mer peptide.Br J Haematol,1995,90:645-654.
  • 5[5]Shattil SJ,Hoie JA,Cunning M,et al.Changes in the platelet membrane glycoprotein Ⅱb-Ⅲa complex during platelet activation.J Biol chem,1985,260:11107-11114.

同被引文献15

  • 1陈秋月,丁美萍,金笑平,林仙方,陈葆国.血小板活化与脑梗死及颈动脉粥样硬化的相关性[J].中国动脉硬化杂志,2004,12(6):704-706. 被引量：6
  • 2马艳,张晓奇,潘荣,贾晓静.稳定型脑梗和进展型脑梗患者血小板膜糖蛋白的对比研究[J].中国老年学杂志,2006,26(7):992-993. 被引量：3
  • 3AbelaG S, Huang R, Ma H, et al. Laser-light scattering, a newmethod for continuous monitoring of platelet acti- vation in circulation fluid [ J ]. Lab ClinMed, 2003,14 (1):5-7.
  • 4Ruggeri ZM. Mechannisms initiating platelet thrombus formation[ J]. Thromb Hae most, 1997,78 ( 1 ) :611.
  • 5Palevsky HI, Kelley MA, Fish man AP. Pulmonary thromboembolic disease. In: Fishman AP ( e d). Fish- man's pulmonary diseases and disorders [ M ]. New York : me GrawHill Co, 1995 : 1297.
  • 6Macchi L, Christiaens L, Braban tS, et al. Resistance to aspirin in vitro is associated with increased platelet sen- sitivity to adenosine ediphosphate [ J ]. Thromb Res, 2002,107(1-2) :4549.
  • 7AbelaG S, huangR, Mall, et al. Laser-light scattering, a newmethod for continuous monitoring of platelet activa- tion in circulation fluid [ J ]. Lab ClinMed, 2003, 14 (1) :5-7.
  • 8Kurabayashi H, Tamuiar J, Naurse T, et al. Possible ex- istence of Platelet activation before the onset of eerebral infraetion [ J ]. Aiheroseleorsis ,2000,153 ( 1 ) : 203-207.
  • 9Yamazaki M, Uehiyama S, Iwata M, et al. Measurement of Platelet fibrinogen binding and P-selectin expression by flow cytometry in patients with eerehral infraction [ J ]. ThrombRes ,2001,104 ( 3 ) : 197-205.
  • 10Michelson AD. Flow cytometry: A clinical test of plate- let funetion [ J ]. Blood, 1996,87:4925-4936.

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陕西医学杂志
2008年 第12期

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