白蛋白固定等离子体聚烯丙胺薄膜及体外血小板粘附行为被引量：2

Study on Plasma Polymerized-allylamine Film Immobilized by Albumin and in vitro Platelet Adhesion Behavior

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摘要采用脉冲射频等离子体聚合技术,在医用不锈钢表面制备了等离子体聚烯丙胺薄膜,并进一步在聚合薄膜表面固定白蛋白(BSA)分子。采用傅立叶红外光谱(FTIR)、X光电子能谱(XPS)、台阶仪、原子力显微镜和淌滴法分别表征结构成分、厚度、表面形貌和水接触角。结果表明:随着放电功率增加,聚烯丙胺薄膜中氮含量及伯胺基浓度减小,薄膜厚度和表面粗糙度增加,接触角增大。XPS分析证明白蛋白分子被固定于聚烯丙胺薄膜表面。体外血小板粘附评价表明,不锈钢表面固定白蛋白的聚烯丙胺薄膜能显著抑制血小板粘附及激活。 In order to improve the anticoagulation of biomedical stainless steel,we fabricated the plasma-polymerized allylamine films on the surface of the biomedical stainless steel by pulsed plasma polymerization technique,and then immobilized the molecules of bovine serum albumin(BSA) on the polyallylamine films.The films were characterized using Fourier transform infrared spectroscopy(FTIR),X-ray photoelectron spectroscopy(XPS),profile meter,atomic force microscopy(AFM) and contact angle measurement.FTIR spectra ...

作者闫峰王进杨志禄张钊孙鸿黄楠

机构地区西南交通大学材料学院

出处《生物医学工程研究》 2008年第4期268-271,280,共5页 Journal Of Biomedical Engineering Research

基金国家自然科学基金资助项目(50673081) 教育部新世纪优秀人才支持计划项目(NCET-07-0715)。

关键词等离子体聚合烯丙胺白蛋白医用不锈钢血小板黏附 Plasma polymerization Allylamine Bovine serum albumin Stainless steel Platelet adhesion

分类号 R318.08 [医药卫生—生物医学工程]

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