双分子荧光互补技术及其在蛋白质相互作用研究中的应用进展

Advances of Bimolecular Fluorescence Complementation Assays and its Application in the Study of Protein-protein Interactions

双分子荧光互补(bimolecular fluorescence complementation,BiFC)是指两个不发光的荧光蛋白互补片段在与其融合的蛋白质的相互作用驱动下重新组装形成荧光复合物,恢复荧光特性。基于双分子荧光互补原理的相关技术在多种不同类型的蛋白质相互作用研究中得到越来越广泛的应用。我们对双分子荧光互补技术的原理、特性、应用现状、面临的问题及应用前景进行了概述。

Bimolecular fluorescence complementation(BiFC) means two non-fluorescent complementary fragments of fluorescent protein can reassemble to form fluorescent complex and restore fluorescence when they are fused to two proteins that interact with each other.BiFC analysis has been used to study interactions among a wide range of proteins in many cell types.In this paper,the principle and characteristics of BiFC,the application advances,the limitation and future prospects of BiFC assays are described.