摘要
目的探讨用凝胶成像分析系统进行DNA定量的DNA含量的检测范围。方法将已知含量的质粒DNA样本稀释成不同含量的DNA样本,进行琼脂糖凝胶电泳。利用凝胶成像系统进行观察及图像采集,并应用IMAGE J软件对DNA的电泳图谱进行灰度分析,用EXCEL软件对已知含量的DNA的灰度值与含量之间进行相关性分析,建立线性方程式。用上述线性方程式计算出样本的DNA含量。结果DNA灰度值与DNA含量之间呈正相关关系,但不成正比关系;DNA灰度值与DNA含量在一定范围内呈现良好的线性关系。通过所建立的线性关系式可以确定DNA含量的检测范围,在此范围内可较好地进行DNA的定量。结论通过建立DNA灰度值与DNA含量之间的良好线性关系,可以较好地确定凝胶成像分析系统进行DNA定量的DNA含量的检测范围。
Objective To investigate the detection range of quantitative measurement of DNA content by gel imaging analysis system.Method The known content of DNA samples were used to make serial dilutions.Electrophoresis of these DNA samples was performed with agarose.The result of electrophoresis was observed and collected by gel an imaging analysis system.The gray scales of DNA sample strip on electrophoregram were analyzed with Image J software.The relationship and linear equation between gray scale of DNA sample s...
出处
《中国体视学与图像分析》
2008年第4期264-267,共4页
Chinese Journal of Stereology and Image Analysis
基金
吉林省科技发展处项目(20050405-7)
