甲基丙烯酰紫草素对肝癌的抑制作用研究

Study on anti-hepatocarcinoma effects of methylacryloylshikonin in vitro and in vivo

目的:研究甲基丙酰紫草素的抗癌作用,并初步探讨其作用机制。方法:MTT法、生长曲线检测甲基丙酰紫草素的体外抗肝癌作用,并计算半数抑制浓度(IC50)值;应用H22荷瘤小鼠模型进行体内抗瘤试验,绘制肿瘤生长曲线并计算抑瘤率;免疫组化方法检测肿瘤组织的凋亡相关蛋白NF-κBp65的表达。结果:甲基丙烯酰紫草素有显著的体外抗癌作用,且与剂量呈正相关,对SMMC-7721的IC50值分别为(21.16±0.96)μg/ml;小鼠移植瘤H22抗瘤试验结果显示,在0.38mg/kg、0.75mg/kg和1.50mg/kg的剂量下甲基丙烯酰紫草素的抑瘤率分别为22.98%、39.08%、47.13%。免疫组化结果表明随甲基丙烯酰紫草素浓度增加NF-κBp65蛋白表达下调。结论:甲基丙烯酰紫草素具有较强的体内外抗肝癌作用,其机制可能与抑制肿瘤细胞增殖、诱导细胞凋亡有关。

Objective :To investigate antitumor effects of methylacryloylshikonin in vitro and vivo.Methods :MTT assay and growth curve assay were used to assess the inhibitory effect of methylacryloylshikonin.Hepatic cancer H22 model in mice was performed to study anti-hepatocarcinoma activity of methylacryloylshikonin in vivo.The growth curve and inhibitory rate of tumor growth was measured.Immunohistochemical assay was used to observe expression of NF-κB P65.Rusults :After treated for 48 hours,MTT assay shows methyl...