姜黄素对急性坏死性胰腺炎大鼠核因子-κB活性的调节

Regulation effect of curcumin on NF-κB activity in rats with ANP

目的:探讨姜黄素对急性坏死性胰腺炎(ANP)大鼠NF-κB活性的调节作用。方法:将SD大鼠随机分为胰腺炎组(ANP组)和姜黄素组(Cur组),通过分次腹腔注射10%L-精氨酸建立大鼠ANP模型,Cur组在造模前20天开始每天给予姜黄素灌胃。两组分别在造模后3h、12h、24h和36h处死大鼠,观察胰腺病理改变、NF-κB活性和血清淀粉酶、MDA、TNF-α、IL-1β的变化。结果:Cur组3h、12h、24h和36h时间点的与ANP组对应时间点比较,胰腺病理改变P=0.049,0.016,0.003,0.001;胰腺组织NF-κB活性P=0.001,0.001,0.006,0.001;淀粉酶P=0.043,0.001,0.002,0.001;丙二醛P=0.026,0.048,0.025,0.000;IL-1βP=0.016,0.016,0.002,0.006;TNF-αP=0.039,0.018,0.005,0.002。结论:姜黄素可能通过抑制NF-κB活性来下调细胞因子水平,并减轻胰腺损伤。

Objective:To explore the effect of curcumine on NF-κB activity with acute necrotizing pancreatitis(ANP)in rats.Method:SD rats were divided into pancreatitis group and curcumine group.All animals were intraperitoneal injected of 10% L-arginine to induce ANP.Curcumin group received curcumine via an intragastric tube daily since day 20 prior to the initiation of ANP.All animals were sacrificed on 3h,12h,24h and 36h after the initiation of ANP to detect pancreas histopathology,NF-κB activity,serum amylase,MDA,I...