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辣椒素诱导白血病和肝癌细胞凋亡的比较研究 被引量：2

Comparative study on apoptosis of leukemia and hepatic tumor cells induced by synthetical capsaicin N-Vanillylnonanamide
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摘要 目的:比较合成辣椒素(N-Vanillylnonanamide)诱导白血病细胞K562及其多药耐药K562/ADM、肝癌HepG2细胞凋亡的作用。方法:以K562细胞、K562/ADM细胞和HepG2细胞为靶细胞,采用噻唑蓝(MTT)比色法检测细胞增殖活性,流式细胞法测定细胞周期和AnnexinⅤ/PI双染色检测细胞凋亡。结果:MTT示合成辣椒素可抑制白血病细胞和HepG2细胞的增殖,根据同时期半数抑制浓度(IC50)示HepG2细胞最敏感,K562细胞较K562/ADM细胞敏感。合成辣椒素可阻滞细胞周期。合成辣椒素诱导24小时后AnnexinⅤ/PI双染色显示白血病细胞和肝癌细胞凋亡率升高;白血病敏感细胞株和耐药细胞株的对合成辣椒素敏感性相似。结论:合成辣椒素可抑制白血病和肝癌细胞的增殖并诱导其凋亡;辣椒素对药物敏感和耐受的白血病细胞的作用相似。 Objective :To compare the apoptosis-inducing effects of synthetical capsaicin(N-Vanillylnonanamide)on leukemia cells and hapatic tumor cells.Methods:The K562 cells,K562/ADM cells and HepG2 cells were used as the target cells,The cell proliferating activity was assessed with a MTT colorimetric assay,the cell cycle was measured using flow cytometry,and the cellular apoptosis was investigated by Annexin V/PI staining.Results:N-Vanillylnonanamide was able to inhibit the proliferation of MTT in leukemia cells an...
作者 陈媛媛 陈静 易娟 魏虎来
机构地区 兰州大学基础医学院医学实验中心甘肃省新药临床前研究重点实验室
出处 《中药药理与临床》 CAS CSCD 北大核心 2008年第6期29-32,共4页 Pharmacology and Clinics of Chinese Materia Medica
基金 甘肃省新药临床前研究重点实验室和甘肃省天然药物重点实验室开放基金资助
关键词 辣椒素 凋亡 白血病 肝癌 N-Vanillylnonanamide apoptosis leukemia hapatic tumor
分类号 R285 [医药卫生—中药学]
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参考文献12

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共引文献59

同被引文献14

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中药药理与临床
2008年 第6期

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