去除IgG的系统性红斑狼疮血小板减少血清对脐带血巨核细胞克隆形成单位生成的影响

Effects of serum without immunoglobulin G from patients with systemic lupus erythematosus associated thrombocytopenia on megakaryocytopoiesis of normal stem cells derived from cord blood

目的:研究去除IgG的系统性红斑狼疮(SLE)合并血小板减少患者的血清,对正常脐带血巨核细胞克隆形成单位(CFU-MK)生成的影响。方法:建立正常人脐带血单个核细胞在血小板生成素(TPO)等作用下,向CFU-MK分化的半固体培养体系;亲和层析去除SLE血小板减少患者血清中的IgG组分并加入培养体系,研究干预前后CFU-MK集落形成的数量和形态学差异。结果:正常脐带血(n=10)CFU-MK集落数为(69.3±19.4)个/片,多为中～大集落;加入SLE血小板减少患者血清(n=4)后,集落数为(58.5±32.5)个/片,与正常相比变化相近(P>0.05),但其中一例患者减少为29个/片;去除血清IgG组分后(n=4),CFU-MK为(73.0±28.3)个/片,与正常相比变化相近(P>0.05);其中一例加入血清后减少为35个/片,去除IgG后仍为31个/片,CFU-MK生成抑制未解除。形态学上,加患者血清后大集落数由正常对照的(37.4±10.9)个/片显著减少为(9.0±8.8)个/片(P<0.05);去除IgG后,大集落数亦显著减少为(8.3±3.9)个/片(P<0.05)。而加入患者血清或去除IgG后,小集落相对数量比正常对照显著增多(P<0.05)。结论:SLE血小板减少患者血清中可能存在阻碍CFU-MK分化发育的抑制物,这种抑制物可能不依赖于血清IgG组分。

AIM:To explore the contribution of serum without immunoglobulin G from patients with systemic lupus erythematosus(SLE) associated thrombocytopenia on megakaryocytopoiesis of stem cells derived from normal cord blood.METHODS:The immunoglobulin G was depleted from serum of patients with SLE associated thrombocytopenia by Affinity Chromatography.The mononuclear cells derived from normal cord blood were cultured in semi-solid culture system in vitro in presence of thrombopoietin(TPO).Colony forming units-megaka...