青蒿琥酯对盲肠结扎穿孔术脓毒症小鼠的保护作用

Protective effects of artesunate on mouse sepsis induced by cecal ligation and puncture

目的:探讨青蒿琥酯(AS)对盲肠结扎穿孔术(CLP)脓毒症小鼠的保护作用及其可能机制。方法:采用CLP制作小鼠脓毒症模型,术后0、4、24、48h重复肌肉注射AS,观察7d内CLP小鼠的死亡率;检测术后4、8h血浆脂多糖(LPS)、TNF-α、IL-6水平;术后8h小鼠肺脏组织形态的变化。结果:AS明显降低CLP小鼠的死亡率(P<0.05)及血浆LPS、TNF-α和IL-6水平(P<0.05或P<0.01),减轻肺组织的损伤。结论:AS对CLP小鼠具有显著的保护作用,该作用可能与其降低血浆LPS水平、减少促炎细胞因子TNF-α、IL-6的释放和减轻组织脏器的损伤有关。

AIM:To investigate the protective effects and related mechanisms of artesunate on mouse model of sepsis induced by cecal ligation and puncture(CLP).METHODS:The septic mouse models were established by CLP.Artesunate was administrated intramuscularly at 0,4,24 and 48 h after CLP.Mortality of CLP mice was observed in the next 7 days.Blood samples were collected at 4,8 h after CLP and the levels of LPS,TNF-α and IL-6 were detected by kinetic turbidimetric limulus test or ELISA,respectively.The morphological cha...