阿托伐他汀对高血压大鼠肾脏血管紧张素转换酶2表达的影响

Effects of atorvastatin on renal angiotensin-converting enzyme 2 expression in hypertensive rats

目的:观察阿托伐他汀对两肾一夹(2K1C)高血压大鼠肾脏血管紧张素转换酶2(ACE2)表达的影响,探讨阿托伐他汀降血压作用可能的新的作用机制。方法:40只雄性Wistar大鼠随机分为5组(每组8只):假手术组、高血压组、缬沙坦组(20 mg·kg-1·d-1)、阿托伐他汀组(10 mg·kg-1·d-1)和阿托伐他汀组(30 mg·kg-1·d-1)。鼠尾容积法测定术前、术后1周及药物干预后4、8周的SBP变化。8周后,免疫组化法检测肾脏ACE2蛋白表达,放射免疫分析法测定心肌组织血管紧张素Ⅱ(AngⅡ)水平,RT-PCR法检测肾脏组织ACE2 mRNA表达。结果:高剂量阿托伐他汀组SBP较高血压组降低显著(P<0.01);高剂量阿托伐他汀组较高血压组降低心肌组织AngⅡ浓度显著(P<0.01);RT-PCR显示缬沙坦组和低、高剂量阿托伐他汀组肾脏组织ACE2 mRNA的表达较高血压组不同程度增高(P<0.05)。结论:2K1C高血压大鼠肾脏组织ACE2蛋白、ACE2 mRNA表达降低。阿托伐他汀在降低高血压大鼠SBP的同时,降低心肌组织AngⅡ浓度,增加肾脏组织ACE2蛋白、ACE2 mRNA表达。

AIM: To observe the effects of atorvastatin on the expressions of renal angiotensin-converting enzyme 2(ACE2) in two kidney and one-clip(2K1C) hypertensive rats and to explore new possible mechanism of atorvastatin in decreasing systolic blood pressure(SBP).METHODS: 40 male Wistar rats were randomly divided into five groups(8 in each group):sham operation group,2K1C hypertension group,2K1C +valsartan group(20 mg·kg-1·d-1),low dose atorvastatin group(10 mg·kg-1·d-1)and high dose atorvastatin group(30 mg·kg-1...