摘要
目的:探讨腺病毒载体介导Fas配体(FasL)基因导入对大鼠颈动脉损伤后血管中层平滑肌细胞密度的影响。方法:实验分治疗组(Ad-FasL组)和正常对照组两组(Ad-βgal组),每组动物均为6只。利用重组腺病毒载体分别将Ad-FasL、Ad-βgal导入大鼠颈动脉球囊损伤的内膜,3、14d后观察其对血管中层平滑肌细胞密度的影响。结果:与正常对照组相比较,通过腺病毒载体介导导入FasL基因的被球囊损伤的大鼠颈动脉,3d后损伤血管中层平滑肌细胞密度降低,14d后血管中层平滑肌细胞密度恢复到正常水平。结论:FasL可抑制血管中层平滑肌细胞密度,从而抑制球囊损伤后的内膜增生。
AIM:To investigate the effects of Fas ligand(FasL)gene transduction mediated by adenoviral vector on the density of medial layer vascular smooth muscle cell after rat carotid artery injury.METHODS:SD rats were divided into treatment group(Ad-FasL group)and normal control group(Ad-βgal group)(n=6).Ad-βgal,Ad-FasL gene were transduced into endomembrane of balloon injury of rat carotid artery using recombination adenovirus vector,the density of medial layer vascular smooth muscle cell was observed after 3,14 d...
出处
《中国临床药理学与治疗学》
CAS
CSCD
2008年第12期1401-1403,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
FAS配体
血管中层平滑肌细胞
腺病毒
基因疗法
Fas ligand
vascular smooth muscle medial cell density
adenovirus vector
gene therapy
