荧光光谱法研究间-硝基苯胺与牛血清白蛋白的相互作用

Spectrometric Study on the Interaction Between m-Nitroaniline and Bovine Serum Albumin

文章采用荧光光谱技术研究了在不同的酸度和温度条件下,间-硝基苯胺与牛血清白蛋白间的相互作用机制.采用荧光猝灭法讨论了不同pH条件下邻硝基苯胺与牛血清白蛋白间的猝灭类型,计算了不同温度和pH条件下的结合常数,并根据热力学参数确定了其主要结合作用力的类型.研究结果表明,间-硝基苯胺对牛血清白蛋白有较强的荧光猝灭作用,符合静态猝灭机理,二者之间的结合作用力主要是范德华力.

The mechanism of interaction between m-nitroaniline and Bovine Serum Albumin(BSA) was investigated with fluorescence spectrometry at different pH and temperature.With fluorescence quenching method,binding constants and thermodynamic parameters were calculated.According to the thermodynamic parameters,the main types of binding forces were determined.The results showed that m-nitroaniline had a powerful ability to quench the BSA fluorescence intensity via a nonradiative energy transfer mechanism,and the main ...