穿心莲有效成分对内皮细胞CD40表达的干预作用

Effect of Andrographitis Paniculata Extracts on the Expression of CD40in Endothelial Cell

目的:检测白细胞分化抗原40(CD40)在动脉内皮细胞中的表达,探讨穿心莲有效成分(API0134)对内皮细胞中CD40表达的干预作用。方法:应用体外细胞培养的方法培养豚鼠主动脉内皮细胞,并将培养出来的内皮细胞分为5组:对照组、氧自由基(OFR)损伤组、氧化型低密度脂蛋白(ox-LDL)损伤组、OFR+API0134组、ox-LDL+API0134组。采用免疫荧光方法以及RT-PCR方法观察并检测ox-LDL及OFR对内皮细胞CD40表达的影响以及API0134对此表达所产生的效应。结果:与对照组相比,ox-LDL及OFR组内皮细胞CD40表达mRNA水平显著增高(P<0.01),而穿心莲组内皮细胞CD40 mRNA表达水平则明显降低(P<0.05);穿心莲组与对应的损伤组比较,内皮细胞上CD40 mRNA的表达水平明显降低(P<0.05)。结论:穿心莲有效成分API0134具有预防动脉粥样硬化作用,其机制可能与其抑制损伤内皮细胞中CD40的表达有关。

Objective: We aimed to investigate the expression of CD40 in endothelial cells (ECs) in a variety of injured conditions and the role of Andrographitis Paniculata isolate (API0134) in endothelial cells. Methods: The thoracic aorta endothelial cells of guinea pig were cultured in vitro until the third passage,incubated in the presence of media containing XO and Xan which produced oxygen free radical (OFR group); oxidized LDL (ox-LDL group); XO,Xan and API0134 (OFR + API0134 group); or ox-LDL and API0134 (ox-L...