胶质细胞源性神经营养因子、Cajal间质细胞和缝隙连接蛋白43在先天性巨结肠中的表达分布研究

Expression and distribution of glial cell derived neurotrophic factor,interstitial cells of Cajal and Connexin 43 in Hirschsprung's disease

目的探讨胶质细胞源性神经营养因子(GDNF)mRNA、Cajal间质细胞(ICC)及缝隙连接蛋白43(Cx43)与先天性巨结肠(HD)发病的关系。方法依据纳入和排除标准选择2006年8月~2007年9月经病理诊断为HD的患儿,取手术切除结肠标本作为HD组,根据取材位置不同分为狭窄段(又分为短段型和常见型)、移行段和扩张段亚组。应用半定量RT-PCR及免疫组化技术检测结肠组织GDNF mRNA水平和ICC、Cx43的分布,以肠套叠患儿手术结肠标本作为对照组。结果研究期间HD组纳入42例,对照组纳入5例。①狭窄段亚组GDNF mRNA表达较扩张段亚组和对照组明显降低(P<0.05);扩张段亚组和对照组GDNF mRNA表达差异无统计学意义(P>0.05)。狭窄段亚组中短段型较常见型GDNF mRNA表达低(P<0.05)。②ICC在对照组和扩张段亚组主要分布于黏膜下丛和肌间丛,呈现连续性分布,相互连接形成网络状结构。ICC在狭窄段亚组结肠组织内的分布显著减少或消失,与对照组和扩张段亚组差异有极显著统计学意义(P<0.001),肌间丛的网络状结构完全破坏,残存ICC形态异常;移行段亚组结肠组织内ICC的分布较对照组...

Objective To investigate the relationship between the gene expression of GDNF and the distribution of interstitial cells of Cajal(ICC),Connexin 43(Cx43) and the mechanism of development of Hirschsprung s disease(HD).Methods Forty-two pairs of full thickness specimens(aganglionic segments and ganglionic segments)were from Hirschsprung s disease patients diagnosed pathologically,immediately after operation.Another five colon specimens were obtained from intussusception patients as control.The GDNF mRNA expres...