摘要
目的:通过检测胃癌细胞系Runx3基因启动子区域甲基化状态及Runx3基因表达情况,探讨Runx3基因启动子区域甲基化在胃癌的发生、发展过程中的意义。方法:采用DNA甲基化特异性PCR(MSP)技术分别对5种胃癌细胞系Runx3基因启动子区域甲基化进行检测,同时采用逆转录-聚合酶链反应(RT-PCR)检测Runx3 mRNA的表达情况;采用DNA甲基化转移酶抑制剂药物5-Aza-CdR(1,3μmol/L)处理胃癌细胞SNU16,并通过RT-PCR检测Runx3 mRNA表达情况。结果:在5种胃癌细胞系中,4种细胞Runx3基因启动子区域过度甲基化及Runx3 mRNA无表达;胃癌细胞SNU16经-Aza-CdR处理后,检测出Runx3基因重新表达。结论:Runx3基因启动子区域甲基化是导致Runx3基因失活的主要原因之一,与胃癌的发生发展密切相关,可作为胃癌早期诊断的分子标记物及分子治疗的靶点。
Objective:To study the clinic significance of Runx3 gene hypermethylation in gastric cancer by examining the promoter methylation status and expression of Runx3 gene in cell lines. Methods:The methylation status of Runx3 was examined by methylation-specific polymerase chain reaction (MSP). Meanwhile,RT-PCR was used to detect expression of Runx3 gene in the gastric carcinoma。 Cell line SNU16 was treated with 5-Aza-CdR (1,3μmol/L),a specific demethylating agent. The change in expression of the Runx3 mRNA in S...
关键词
胃肿瘤
RUNX3基因
甲基化
细胞系
甲基化特异性PCR
逆转录-聚合酶链反应
Gastric cancer
Runx3 gene
Methylation
Cell lines
Methylation-specific polymerase chain reaction （MSP）
Reverse transcription-polymerase chain reaction（RT-PCR）
