摘要
目的探讨应激后新生鼠小肠上皮细胞线粒体功能损害及褪黑素对肠道的保护作用。方法健康5～7dSD大鼠新生鼠,随机分成对照组(C组)、手术组(S组)、假手术组(F组)、褪黑素组1(M1组)和褪黑素组2(M2组)。电镜观察肠上皮细胞线粒体形态变化。测定线粒体丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide dismutase,SOD)、一氧化氮(nitric oxides,NO)的含量变化。结果手术组和假手术组与对照组相比MDA的水平升高,SOD和NO的水平降低,线粒体肿胀明显,嵴出现扭曲、疏松溶解;褪黑素组1和褪黑素组2与手术组和假手术组相比MDA的水平显著降低,SOD水平升高,线粒体的水肿和嵴的破坏减轻。结论线粒体损伤是外科应激后新生鼠小肠上皮细胞肠道损伤的早期表现。褪黑素能明显减轻外科应激对小肠上皮细胞线粒体的损害,保护应激后肠道功能。
Objective To study enterocyte mitochondrial dysfunction of neonatal rats undergoing surgical stress and the protective effects of melatonin.Methods 30 Sprague-Dawley neonatal rats were randomly divided into 5 groups: control(no operation),surgical manipulation(intestinal handling),sham control(laparotomy without intestinal handling),melatonin treated groups 1(M1),melatonin treated groups 2(M2).Mitochindrial ultrastructure and parameters of morphology of the above groups were observed by electron microscope....
出处
《中国血液流变学杂志》
CAS
2008年第4期489-490,571,共3页
Chinese Journal of Hemorheology
基金
江苏省卫生厅医学重点人才基金项目(RC2003098)
关键词
小肠
外科应激
线粒体
褪黑素
intestine
surgical stress
mitochondria
melatonin
