高效液相色谱法测定山楂中金丝桃苷的含量

The determination of hyperoside in Fructus Crataegi by HPLC

目的:建立山楂中金丝桃苷的含量测定方法。方法:采用高效液相色谱法测定,选定ODS-C18色谱柱(4.6mm×150mm,5μm),流动相:甲醇-水-冰醋酸(41∶58∶1,v/v/v),检测波长:355nm。结果:本法线性范围为1.032～20.64μg/ml(r=0.9999),平均回收率为99.0%(n=9),RSD=0.60%。结论:该方法简便、灵敏、准确、重现性好,可用于山楂的质量控制。

Objective:To establish a determination method of hyperoside in Fructus Crataegi.Method:HPLC,column:ODS-C18(4.6 mm×150 mm,5 μm),mobile phase:methanol-water-glacial acetic acid(41∶58∶1,v/v/v),wavelength:355 nm.Result:The linear range was 1.032～20.640 μg/ml(r=0.999 9)and recovery rate was 98.99%(n=9),RSD=0.60%.Conclusion:This method is convenient,sensitive and accurate.And it can be used as standard quality control of Fructus Crataegi.