摘要
为探讨爪哇伪枝藻胞外多糖(Extracellular polymeric substances ofScytonema javanicum,EPS)诱导人表皮癌A431细胞凋亡及其对凋亡相关基因caspase-3、bcl-2和bax表达的影响,本实验利用MTT法检测细胞生长抑制情况;HE染色法及透射电镜进行形态学观察;单细胞凝胶电泳法(SCGE/彗星电泳)分析DNA受损情况;免疫组织化学法检测细胞内caspase-3、bcl-2和bax表达水平。结果显示EPS能显著抑制A431细胞增殖,并呈时间和剂量依赖性,作用96h的半数抑制浓度IC50为4.25mg/mL,并出现细胞凋亡的形态学改变;彗星电泳结果与对照相比6mg/mL EPS作用48h能引起A431细胞DNA严重损伤;免疫组织化学检测发现6mg/mLEPS作用72h能显著上调A431细胞内凋亡相关基因caspase-3和bax的表达,而下调bcl-2的表达。
To investigate the effect of extracellular polymeric substances of Scytonema javanicum(EPS) on apoptosis of A431 cells and the expression level of relative genes,cell viability was measured by MTT method,morphologic changes were observed by hematoxylin and eosin staining and transmission electron microscopy,DNA damage was detected by single cell gel electrophoresis(SCGE/comet assay),the expression level of caspase-3,bcl-2 and bax were detected by immunohistochemical staining.EPS significantly inhibited the ...
出处
《水生生物学报》
CAS
CSCD
北大核心
2008年第6期874-880,共7页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(3077039530370112)
国家载人航天工程计划
内蒙古发改委与武汉市科技局重大攻关计划
中国科学院水生所领域前沿项目资助
关键词
爪哇伪枝藻
胞外多糖
A431
细胞凋亡
Scytonema javanicum
Extracellular polymeric substances
A431
Apoptosis
