蛋白质酶解混合物的动态修饰毛细管电泳-质谱联用分析

Analysis of Protein-digested Mixture with Dynamic Coating Capillary Electrophoresis-Mass Spectrometry

利用羟丙基纤维素溶液动态涂层技术修饰毛细管管壁 ,改善了分离效率 .在不影响质谱检测的条件下 ,将动态涂层毛细管电泳与质谱检测联用 ,有效地提高了对蛋白质的鉴定能力 .将该技术应用于对复杂蛋白质样品的酶解产物的分析鉴定 。

An improved method of mass compatible dynamic coating was developed for proteins identification with capillary electrophoresis-mass spectrometry(CE/MS), which kept the high separation efficiency of dynamic coating and eliminated the negative effects of coating reagent to MS detection. The identification efficiency of protein by the peptide mapping of tryptic digested protein was improved. The complex protein mixture was analyzed and successfully identified by this method. The identification efficiency of protein by the peptide mapping to tryptic digested protein was dramatically improved, that the MAscot score of myoglobin digest analysis on CE/MS was increased from 304 to 914 with the dynamic coating. Four protein tryptic digests mixture was also analyzed and successfully identified by this method.