基于复合型微流控芯片的紫外检测毛细管电泳系统

A Hybrid Microfluidic Chip-based Capillary Electrophoresis System with UV Absorbance Detection

提出了一种基于芯片 -毛细管复合装置的紫外检测 -微流控芯片毛细管电泳分析系统 .采用小死体积的耦合技术实现了石英毛细管与“十”字通道型微流控玻璃芯片的耦合 .本系统的紫外检测灵敏度与商品化毛细管电泳仪相当 .采用夹流进样方式 ,达到较高的进样重现性 ,2 mmol/L苯甲酸的峰高相对标准偏差 ( RSD)为 1 .5 % ( n=1 1 ) .

A hybrid microfluidic system produced by coupling a microfabricated glass chip with conventional silica capillary for UV absorbance detection was developed. The system has proved to be robust, and easily fabricated at a low cost. The effects of sample loading and separation field strength on plate number, migration time and signal response were observed in the optimization studies. The reproducibility was examined by using benzoic acid as a model sample and following similar behavior as for complete photolithographically produced glass chip systems. The results showed that the reproducibility is good and the RSD(n=11) was only 1.5% for benzoic acid. The performance of the system was demonstrated by separating sulphamethoxazole(SMZ) and trimethoprim(TMP) in sulphatrim tablets within 70 s. The detection limits were comparable to those obtained with a commercial capillary electrophoresis instrument.