TSA诱导人粘液表皮样癌细胞MEC-1凋亡机制的初步研究

An experimental study on the apoptosis of human mucoepidermoid carcinoma cell MEC-1 induced by Trichostatin A

目的探讨曲古抑菌素A(TSA)对人粘液表皮样癌细胞(MEC-1)的体外生物学作用及其作用机制。方法应用不同浓度的TSA作用于人粘液表皮样癌细胞MEC-1细胞后,观察TSA对MEC-1细胞生长状态的影响;通过噻唑蓝(MTT)比色法检测TSA对MEC-1细胞增殖变化的作用,应用原位末端标记法(TUNEL)、流式细胞术检测经Annexin V-FITC/PI双染后MEC-1细胞的早期凋亡作用。结果TSA可显著地抑制MEC-1细胞的生长,并且具有时间和剂量依赖性,通过倒置显微镜可明显观察到细胞的形态学改变;通过TUNEL法、流式细胞仪检测显示TSA可以诱导MEC-1细胞凋亡。结论TSA对人粘液表皮样癌细胞MEC-1具有显著的体外生长抑制,其主要作用是诱导细胞凋亡。

Objective To investigate the biological effects of Trichostatin A(TSA) and its mechanism on human mucoepidermoid carcinoma cell MEC-1 in vitro.Methods After treatment with TSA of different concentrations(0.1μg/ml～0.8μg/ml),the effects of TSA on the growth of human mucoepidermoid carcinoma cell MEC-1 was observed.The inhibitory effect of TSA on the cell proliferation was investigated with 3-(4,5-dimethyl-thizazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) method and cell apoptosis was detected by TUNEL and ...