三叉神经痛患者患支神经中hNa_V 1.8钠通道蛋白的定量表达

The quantitative expression of hNa_V 1.8 sodium channel protein in affected nerves of patients with trigeminal neuralgia

目的通过研究河豚毒素不敏感型(tetrodotoxin-resistant,TTX-R)hNaV 1.8钠通道蛋白在三叉神经痛(trigeminal neuralgia,TN)患者痛支神经中的表达水平,探讨TN的发病机制和为TN的药物治疗提供新的靶标。方法以12例原发性TN第三支痛保守治疗无效行手术治疗患者的下牙槽神经、舌神经为研究对象,以舌颌颈联合根治术患者的正常下牙槽神经1例为阴性对照,肌肉组织1例为正常对照,鼠背根神经节为阳性对照,与制备的hNav 1.8钠通道蛋白抗体进行免疫组化反应,并对两组免疫组化结果进行定量分析,研究hNaV 1.8钠通道蛋白在TN患者患支中的表达水平。结果hNaV 1.8钠通道蛋白在TN患者患支神经中均存在表达,且痛支神经中的表达多于非痛支神经(P<0.01),而正常下牙槽神经及肌肉组织中无表达。结论hNaV 1.8钠通道蛋白在TN患者痛支中的异常表达可能与TN的发病机制有密切关系。

Objective To understand the pathogenesis of trigeminal neuralgia(TN) and to provide a new target for the medicine treatment of TN by studying the expression of Tetrodotoxin-resistant hNaV1.8 sodium channel protein in affected nerves of patients with TN.Methods Twelve affected inferior alveolar nerves and lingual nerves obtained from patients with idiopathic trigeminal neuralgia,and without effective for the medicine therapy,were studied.As negative control,one normal inferior alveolar nerve was obtained fro...