摘要
目的探讨长链多不饱和脂肪酸对体外培养的成年海马神经祖细胞的增殖及突起生长影响。方法使用从成年大鼠海马分离培养的神经祖细胞,分别经花生四烯酸(AA)、二十碳五烯酸(EPA)或二十二碳六烯酸(DHA)处理后,以乳酸脱氢酶(LDH)分析法测定细胞活性;再行免疫细胞荧光染色后做突起的定量测量。结果EPA、DHA和AA在高浓度时均显示使细胞数显著增加的趋势(P<0.05),但只有EPA和DHA使细胞突起长度显著增长(P<0.05)。结论长链多不饱和脂肪酸能促进神经祖细胞增殖,其中ω-3脂肪酸还能促进细胞突起长度的增长。
Objective To explore the effect of long-chain polyunsaturated fatty acids on the proliferation and process growth of the neural progenitor cells.Methods Cultured neural progenitor cells isolated from adult rat hippocampus were used. After arachidonic acid (AA),eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) treatment respectively,cell numbers were determined by Lactate dehydrogenase (LDH) assay,and the quantitative measurements of the cell processes were done after the fluorescent immune cell stai...
出处
《脑与神经疾病杂志》
2009年第1期17-19,共3页
Journal of Brain and Nervous Diseases
基金
日本文部科学省学术振兴会科研费资助项目(18390324)
关键词
长链多不饱和脂肪酸
花生四烯酸
二十碳五烯酸
二十二碳六烯酸
神经祖细胞
Long-chain polyunsaturated fatty acids
Arachidonic acid （AA）
Eicosapentaenoic acid （EPA）
Docosahexaenoic acid （DHA）
Neural progenitor cells
