脾虚模型脑内C-fos水平与C-JUNmRNA表达变化及归脾汤的影响

Changes of C-fos Evel and C-JUN Gene Expression in Brain of Spleen-Deficiency Rat Model and Influence of Guipi Decoction

目的:研究脾虚模型脑内即早基因C-fos水平与C-JUNmRNA表达变化及归脾汤的影响。方法:采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,免疫组化法、原位杂交法检测下丘脑腹侧核、海马CA1区、前额叶皮层C-fos水平与C-JUNmRNA表达变化及归脾汤的治疗作用。结果:模型组C-fos免疫阳性反应物与C-JUNmRNA表达在下丘脑腹侧核、海马CA1区、前额叶皮层明显降低;治疗组上述脑区的C-fos免疫阳性反应物C-JUNmRNA表达明显增加。结论:脾虚模型脑内C-fos水平与C-JUNmRNA表达有变化,提示归脾汤可能通过影响C-fos水平与C-JUNmRNA表达而调节学习记忆功能。

Objective:To observe the level changes of C-fos and C-JUN gene expression in the brain of spleen-deficiency rat model and The influence of Guipi Decoction. Methods:To establish the rat model of spleen-deficiency by the methods of purgation with bitter and cold nature dyugs, omproper diet and overstrain. Immunohistochemistr and in situ hybridization were used to tetect and C-fos and C-JUNmRNA expression in the hypothalamus ventral kernel, the hippocampi CA1 area and forehead cortex. Result: The immunity posi...